%0 Conference Proceedings
%T A Restoration and Extrapolation Iterative Method for Band-limited Fluorescence Microscopy Image
%D 2007
%A Ponti Junior, Moacir P.,
%A Mascarenhas, Nelson D. A.,
%A Suazo, Claudio A. T.,
%@affiliation Departamento de Computação - Universidade Federal de São Carlos
%@affiliation Departamento de Computação - Universidade Federal de São Carlos
%@affiliation Departamento de Engenharia Química - Universidade Federal de São Carlos
%E Falcão, Alexandre Xavier,
%E Lopes, Hélio Côrtes Vieira,
%B Brazilian Symposium on Computer Graphics and Image Processing, 20 (SIBGRAPI)
%C Belo Horizonte
%8 Oct. 7-10, 2007
%S Proceedings
%I IEEE Computer Society
%J Los Alamitos
%K Image Restoration, Super-resolution, Fluorescence Microscopy.
%X Optical microscopic images, especially with a nonconfocal microscope, are fundamentally limited because the optical transfer function (the Fourier transform of the point-spread function) is zero over a region of the spatial-frequency domain. Iterative algorithms were developed for the restoration and extrapolation of diffraction-limited imagery. In this paper we present the effectiveness of an iterative method based on the Richardson-Lucy algorithm for image restoration and a simultaneous modified version of Gerchberg-Papoulis method to extrapolate the spectrum and control the noise amplification. Good convergence stabilization results were achieved and also good numerical results were observed.
%@language en
%3 PontiJunior-RestorationExtrapolation_2007.pdf