Identity statement area
Reference TypeConference Paper (Conference Proceedings)
Last Update2015: (UTC)
Metadata Last Update2020: (UTC) administrator
Citation KeyGregóriodaSilvaCarvFerr:2015:DeLeIn
TitleDetection of leukocytes in intravital video microscopy based on the analysis of Hessian matrix eigenvalues
Access Date2021, Dec. 07
Number of Files1
Size3040 KiB
Context area
Author1 Gregório da Silva, Bruno César
2 Carvalho-Tavares, Juliana
3 Ferrari, Ricardo José
Affiliation1 Federal University of São Carlos
2 Federal University of Minas Gerais
3 Federal University of São Carlos
EditorPapa, João Paulo
Sander, Pedro Vieira
Marroquim, Ricardo Guerra
Farrell, Ryan
Conference NameConference on Graphics, Patterns and Images, 28 (SIBGRAPI)
Conference LocationSalvador
DateAug. 26-29, 2015
PublisherIEEE Computer Society
Publisher CityLos Alamitos
Book TitleProceedings
Tertiary TypeFull Paper
History (UTC)2015-06-19 21:01:40 :: -> administrator ::
2020-02-19 02:14:03 :: administrator -> :: 2015
Content and structure area
Is the master or a copy?is the master
Content Stagecompleted
Content TypeExternal Contribution
Keywordsdetection of leukocytes
intravital microscopy
temporal image registration
AbstractDetection of rolling and adhered leukocytes in intravital microscopy image sequences is an important task in studies of leukocyte-endothelial interactions in the microcirculation of living small animals under different inflammatory conditions. This procedure is usually performed by visual assessment of the image sequences. However, despite being tedious and time consuming, this procedure is prone to the inter- and intra-observer variability. In this work, we developed an automated computer system for the detection of leukocytes in intravital video microscopy. First, the video frames were processed by the bilateral filter to reduce noise while preserving sharp edges. Then, a demons-based image registration technique was applied to minimize animal motion. Finally, the detection of leukocytes was performed by local analysis of Hessian matrix eigenvalues. Quantitative and qualitative evaluation of the proposed method were conducted by using 220 video frames obtained from an experimental study performed on the brain microvasculature of mice. The proposed method was compared with the template matching technique using precision, recall and F1-Score measures. For the Hessian-based method, the results of precision, recall and F1-Score were, respectively, equal to 0.81, 0.86, and 0.83. For direct comparison, the results obtained for the template matching technique were 0.86, 0.73 and 0.79. > SDLA > SIBGRAPI 2015 > Detection of leukocytes...
doc Directory Contentaccess
source Directory Contentthere are no files
agreement Directory Content
agreement.html 19/06/2015 18:01 0.7 KiB 
Conditions of access and use area
data URL
zipped data URL
Target Filemanuscript_sibigrapi_2015.pdf
Update Permissionnot transferred
Allied materials area
Next Higher Units8JMKD3MGPBW34M/3K24PF8
Notes area
Empty Fieldsaccessionnumber archivingpolicy archivist area callnumber copyholder copyright creatorhistory descriptionlevel dissemination doi edition electronicmailaddress group holdercode isbn issn label lineage mark nextedition notes numberofvolumes orcid organization pages parameterlist parentrepositories previousedition previouslowerunit progress project readergroup readpermission resumeid rightsholder secondarydate secondarykey secondarymark secondarytype serieseditor session shorttitle sponsor subject tertiarymark type url versiontype volume